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Merck

SAB4500003

Anti-BCL-2 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, BCL2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00239644
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IF, IHC, WB
Citations:
34

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 26 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

NCBI accession no.

P10415

UniProt accession no.

P10415

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BCL2(596)

General description

B-cell lymphoma 2 (BCL-2) is an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein. It is a target gene of nuclear factor kappa B (NF-kB). The gene encoding BCL-2 is localized on human chromosome 18q21.

Immunogen

The antiserum was produced against synthesized peptide derived from human BCL-2.

Immunogen Range: 46-95

Application

Anti-BCL-2 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

BCL2 is an apoptosis regulator that is expressed in sub-cellular components such as the mitochondria and nucleus. While mitochondrial BCL2 inhibits apoptosis, BCL2 present in the nucleus may activate cell death. BCL2 may also regulate autophagy during starvation . Anti-BCL-2 antibodies are specific for endogenous levels of total BCL-2 protein in rats, humans and mice.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
424170774
210774

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Bcl-2 is a critical mediator of intestinal transformation.
Heijden M, et al.
Nature Communications (2016)
Different signalling in infarcted and non-infarcted areas of rat failing hearts: A role of necroptosis and inflammation
Lichy M, et al.
Journal of Cellular and Molecular Medicine (2019)
Bcl-2 localized at the nuclear compartment induces apoptosis after transient overexpression.
Portier BP
The Journal of Biological Chemistry (2006)
Xuan-Pei Xu et al.
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 22(1), 58-64 (2020-01-18)
To study the effect and mechanism of action of irisin on hypoxic-ischemic brain damage in neonatal rats. A total of 248 7-day-old Sprague-Dawley rats were randomly divided into a sham-operation group, a model group, and low- and high-dose irisin intervention
Analysis of necroptotic proteins in failing human hearts
Szobi A, et al.
Journal of Translational Medicine (2017)
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