SML0569

RO-3306

Name: RO-3306
Brand: SIGMA

≥98% (HPLC), powder, CDK1 inhibitor

Synonym(s):

(5Z)-5-Quinolin-6-ylmethylene-2-[(thiophen-2-ylmethyl)-amino]-thiazol-4-one, 5-(6-Quinolinylmethylene)-2-[(2-thienylmethyl)amino]-4(5H)-thiazolone

Select a Size

All Photos(3)

About This Item

Empirical Formula (Hill Notation):

C₁₈H₁₃N₃OS₂

CAS Number:

872573-93-8

Molecular Weight:

351.45

UNSPSC Code:

12352200

PubChem Substance ID:

329825559

NACRES:

NA.77

MDL number:

MFCD17392573

Product Name

RO-3306, ≥98% (HPLC)

SMILES string

O=C1N=C(NCC2=CC=CS2)S/C1=C\C3=CC4=C(C=C3)N=CC=C4

InChI key

XOLMRFUGOINFDQ-YBEGLDIGSA-N

InChI

1S/C18H13N3OS2/c22-17-16(24-18(21-17)20-11-14-4-2-8-23-14)10-12-5-6-15-13(9-12)3-1-7-19-15/h1-10H,11H2,(H,20,21,22)/b16-10-

assay

≥98% (HPLC)

form

powder

color

off-white to brown

solubility

DMSO: 5 mg/mL, clear

storage temp.

2-8°C

Quality Level

100

Related Categories

Bioactive Small Molecule Inhibitors

Bioactive Small Molecules

Application

RO-3306 has been used:

To study the significance of CA4-mediated cytotoxicity in mitotic arrest
In cell cycle synchronization to conduct a study on proteomics
As a CDK1 inhibitor, to prevent early mitotic entry

Biochem/physiol Actions

CDK1 (cyclin dependent kinase 1) is considered to be the “master switch” in cell division, which maintains the mitotic state of cells.

RO-3306 is a selective ATP-competitive inhibitor of CDK1.

RO-3306 is a selective ATP-competitive inhibitor of CDK1. It inhibites CDK1 cyclin B1 activity with Ki of 35 nM, nearly 10-fold selectivity relative to CDK2/cyclin E and over 50-fold relative to CDK4/cyclin D. RO-3306 has been used to cause cell cycle arrest at the G2/M boundary.

Features and Benefits

This compound is featured on the CDKs page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Increased activity of both CDK1 and CDK2 is necessary for the combinatorial activity of WEE1 inhibition and cytarabine.

Tamara B Garcia et al.

Leukemia research, 64, 30-33 (2017-11-28)

Inhibition of WEE1 is emerging as a promising chemosensitization strategy in many cancers including acute leukemia. Our lab and others have demonstrated that a small-molecule inhibitor of WEE1, AZD1775, sensitizes acute leukemia cells to cytarabine; however, a mechanism of combinatorial

Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1.

Vassilev L T, et al.

Proceedings of the National Academy of Sciences of the USA, 103(28), 10660-10665 (2006)

Visualization of human karyopherin beta-1/importin beta-1 interactions with protein partners in mitotic cells by co-immunoprecipitation and proximity ligation assays.

Francesco L, et al.

Scientific Reports, 8(1), 1850-1850 (2018)

Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest.

Tarade D, et al.

PLoS ONE, 12(3), e0171806-e0171806 (2017)

BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination.

Feng W and Jasin M

Nature Communications, 8(1), 525-525 (2017)

View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service