Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.21
UNSPSC Code:
41105500
Assay:
≥85% (HPLC)
Form:
solution
Storage temp.:
−20°C
assay
≥85% (HPLC)
form
solution
mol wt
(Mr = 1106.7 (DIG-11-UTP-Li4))
packaging
pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])
manufacturer/tradename
Roche
storage temp.
−20°C
General description
Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.
Application
Digoxigenin-11-UTP has been used for labeling RNA probes in:
- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
Biochem/physiol Actions
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
Analysis Note
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Other Notes
For life science research only. Not for use in diagnostic procedures.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
<BIG>Chen X, et al.</BIG>
Biotechniques, 56 (2014)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)