806552
Phosphate Buffered Saline
pH 7.4, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Phosphate buffered saline, PBS
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About This Item
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
pH
7.4
General description
Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
Application
Phosphate-buffered saline has been used:
- as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
- has also been used to wash cells during immunofluorescence staining
- as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Shangang Zhao et al.
Molecular metabolism, 37, 100995-100995 (2020-04-15)
Hyperleptinemia per se is sufficient to promote leptin resistance in the obese state. Leptin sensitivity can be restored by reducing circulating leptin levels within a physiologically healthy range and is a viable antiobesity and antidiabetic strategy. However, a previous study
Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
Tavakol S, et al.
Molecular Neurobiology (2016)
Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable