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Merck

A5955

Antibiotic Antimycotic Solution (100×), Stabilized

suspension, suitable for cell culture, BioReagent

Synonym(s):

Penicillin-Streptomycin-Amphotericin B Suspension

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76
MDL number:
MFCD00130520

Product Name

Antibiotic Antimycotic Solution (100×), Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered, BioReagent, suitable for cell culture

sterility

0.1 μm filtered

product line

BioReagent

form

suspension

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
fungi
yeast

application(s)

agriculture

mode of action

cell wall synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Application

Antibiotic antimycotic solution has been used:
  • as a supplement in different types of cell culture media.
  • as a component of phosphate buffer saline (PBS) sterile solution to prevent contamination of periodontal ligament fibroblasts (PDLF) tissues that are isolated and cultured in the experiments.
  • as a component of storage solution for preserving tracheal segments harvested for subsequent experimental investigation.

Biochem/physiol Actions

Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out.

Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.

General description

Antibiotic Antimycotic Solution is formulated to contain 10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and is solubilized in a proprietary citrate buffer. It is recommended for use in cell culture applications at 10mL/L. Antibioticantimycotic solution is generally used to prevent the growth of harmful microorganisms including bacteria and fungi in cell culture media.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000488860
0000488859
0000488859
0000488860
0000478533

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Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols
Bioengineering (Basel, Switzerland), 10(5) (2023)
Monireh Kouhi et al.
Journal of biomedical materials research. Part A, 107(6), 1154-1165 (2019-01-13)
Guided bone regeneration (GBR) has been established to be an effective method for the repair of defective tissues, which is based on isolating bone defects with a barrier membrane for faster tissue reconstruction. The aim of the present study is
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Classification of morphological features in biological samples is usually performed by a trained eye but the increasing amount of available digital images calls for semi-automatic classification techniques. Here we explore this possibility in the context of acrosome morphological analysis during
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Global Trade Item Number

SKUGTIN
A5955-20ML04061838447012

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