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Merck

C2885

CHES

≥99.0% (titration)

Synonym(s):

2-(Cyclohexylamino)ethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
103-47-9
Molecular Weight:
207.29
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24892541
EC Number:
203-115-6
Beilstein/REAXYS Number:
2967601
MDL number:
MFCD00003835

Key Documents

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Quality Level

200

assay

≥99.0% (titration)

form

crystalline

pH

3.0-5.0 (103.6 g/L)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

water: 0.6 g/25 mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:
  • as a buffer in mannanase activity assay of antarctic fungal isolates
  • as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
  • as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000541111
0000541112
0000498793
0000532391
0000532392

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Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods
Gujska E and Kuncewicz A
European Food Research and Technology, 221(1-2), 208-213 (2005)
Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
Taha M, et al.
Green Chemistry, 16(6), 3149-3159 (2014)
Detection of Bulky Endogenous Oxidative DNA Lesions Derived from 8, 5?-Cyclo-2?-deoxyadenosine by 32P-Postlabeling Assay
Zhou GD and Moorthy B
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 64(1), 17-17 (2015)
Scott A Sarver et al.
Journal of chromatography. A, 1229, 268-273 (2012-02-11)
Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-
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