C6198
Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclonal
clone 1A4, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Actin, α-Smooth Muscle, Alpha-actin 2 Cy3, alpha-smooth muscle actin - Cy3™ antibody, SMA
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Product Name
Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclonal, clone 1A4, purified from hybridoma cell culture
biological source
mouse
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1A4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
isotype
IgG2a
UniProt accession no.
application(s)
research pathology
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
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Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Biochem/physiol Actions
Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin.
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
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