G6048
Galactokinase human
recombinant, expressed in E. coli
Product Name
Galactokinase human, recombinant, expressed in E. coli
recombinant
expressed in E. coli
form
solution
specific activity
≥1400 unit/μg protein
mol wt
42 kDa
shipped in
dry ice
storage temp.
−70°C
Quality Level
Biochem/physiol Actions
Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway.
General description
N-terminal GST-tagged 42 kDa full length protein
Other Notes
One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.
Physical form
Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dariusz Abramczyk et al.
Eukaryotic cell, 11(3), 334-342 (2012-01-03)
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and
Shivani Malik et al.
Nucleic acids research, 40(8), 3348-3363 (2011-12-27)
Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes
Nils Janzen et al.
Archives of medical research, 42(7), 608-612 (2011-12-14)
Galactokinase (GALK) deficiency is an autosomal recessive disorder causing cataract formation that can be prevented or mitigated by early diagnosis and galactose-restricted diet. The aim of this retrospective study was to explore whether GALK-deficiency meets the criteria for neonatal mass
Tali Lavy et al.
Genes & development, 26(3), 294-303 (2012-02-04)
A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. Galactose induces this transcriptional switch, which is regulated by three proteins:
P A Frey
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 10(4), 461-470 (1996-03-01)
The biological interconversion of galactose and glucose takes place only by way of the Leloir pathway and requires the three enzymes galactokinase, galactose-1-P uridylyltransferase, and UDP-galactose 4-epimerase. The only biological importance of these enzymes appears to be to provide for
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