S6258
Spectinomycin ready made solution
100 mg/mL in DMSO/H2O, 1:1, Cell culture tested
Synonym(s):
Actinospectacin, Espectinomicina, Spectacin, Spectam, Togamycin, Trobicin
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About This Item
NACRES:
NA.76
UNSPSC Code:
12352207
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General description
Spectinomycin is an aminocyclitol antibiotic naturally produced by Streptomyces spectabilis. Spectinomycin shows in vitro inhibitory effect (31.2μg/mL) against gram-negative and gram-positive bacteria like Escherichia coli, Klebsiella, Enterobacter, and Staphylococcus epidermidis. It acts as a protein synthesis inhibitor by binding to the bacterium′s 30S ribosomal subunit. Specifically, it interacts with 16S rRNA during the elongation process and blocks elongation factor G (EF-G), which is responsible for the translocation catalysis of peptidyl-tRNA.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Physical form
Liquid - in 1:1 DMSO:water
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Alain Brans et al.
Applied and environmental microbiology, 70(12), 7241-7250 (2004-12-03)
The novel method described in this paper combines the use of blaI, which encodes a repressor involved in Bacillus licheniformis BlaP beta-lactamase regulation, an antibiotic resistance gene, and a B. subtilis strain (BS1541) that is conditionally auxotrophic for lysine. We
Y H Kouri et al.
Genitourinary medicine, 65(5), 342-346 (1989-10-01)
Efficacy of single-dose spectinomycin (TRO: 2 g intramuscularly) was compared with that of aqueous procaine penicillin G (APPG:4.8 x 10(6) units) plus 1 g of probenecid for treatment of gonococcal urethritis and cervicitis. Cure rates of the 210 patients who
Hélène Louvel et al.
Current protocols in microbiology, Chapter 12, Unit 12E-Unit 12E (2008-09-05)
The genus Leptospira belongs to the order Spirochaetales and is composed of both saprophytic and pathogenic members, such as Leptospira biflexa and L. interrogans, respectively. A major factor contributing to our ignorance of spirochetal biology has been the lack of
Z Svab et al.
Plant molecular biology, 14(2), 197-205 (1990-02-01)
The bacterial gene aadA encodes the enzyme aminoglycoside-3"-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or
A Bollen et al.
Science (New York, N.Y.), 165(3888), 85-86 (1969-07-04)
Reconstitution of 30S ribosomal particles from 16S ribosomal RNA and total proteins, or from core proteins and split proteins obtained from the ribosomes of strains of Escherichia coli sensitive to and resistant to spectinomycin, shows that the split protein fraction
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