SRE0026
Phosphatase substrate
Suitable for manufacturing of diagnostic kits and reagents
Synonym(s):
Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Quality Level
assay
≥97% (HPLC)
form
powder
solubility
water: 100 mg/mL, clear to slightly hazy
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Still not finding the right product?
Explore all of our products under Phosphatase substrate
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Single step, direct fluorescence immunoassays based on metal enhanced fluorescence (MEF-FIA) applicable as micro plate-, array-, multiplexing-or point of care-format
Hawa G, et al.
Analytical biochemistry, 549, 39-44 (2018)
Cheng-Chia Tang et al.
eLife, 10 (2021-06-24)
Bone formation and resorption are typically coupled, such that the efficacy of anabolic osteoporosis treatments may be limited by bone destruction. The multi-kinase inhibitor YKL-05-099 potently inhibits salt inducible kinases (SIKs) and may represent a promising new class of bone
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the