U1500
Urease from Canavalia ensiformis (Jack bean)
Type III, powder, 15,000-50,000 units/g solid
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
15,000-50,000 units/g solid
Biological source:
Canavalia ensiformis
biological source
Canavalia ensiformis
type
Type III
form
powder
specific activity
15,000-50,000 units/g solid
mol wt
~544620 Da
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Quality Level
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General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.
Biochem/physiol Actions
Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.
Other Notes
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
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In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary
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Current medicinal chemistry, 26(1), 216-231 (2017-10-11)
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