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Merck

12072

Benzamidine

≥95.0%

Sinónimos:

Benzenylamidine, Phenylamidine

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About This Item

Fórmula lineal:
C6H5C(=NH)NH2
Número CAS:
Peso molecular:
120.15
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
210-546-3
Beilstein/REAXYS Number:
606020
MDL number:

Nombre del producto

Benzamidine, ≥95.0%

InChI key

PXXJHWLDUBFPOL-UHFFFAOYSA-N

InChI

1S/C7H8N2/c8-7(9)6-4-2-1-3-5-6/h1-5H,(H3,8,9)

SMILES string

NC(=N)c1ccccc1

assay

≥95.0%

form

powder or crystals

functional group

amine
phenyl

Quality Level

Application

Benzamidine was used in the preparation of heparan sulfate-rich proteoglycan from mouse mammary epithelial cells. It has been used to prevent enzymatic degradation during the purification procedure of bovine factor XII (Hageman factor).

Biochem/physiol Actions

Benzamidine is an inhibitor of plasmin and has been used in the radioimmunoassay of glucagon in human plasma. It prevents the destruction of Thyrotropin-releasing hormone (TRH) added to whole blood in vitro before radioimmunoassay of TRH.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What solvents can be used to dissolve Benzamidine, Product 12072?

    Based on our experience with this compound in quality control, we would expect it to be soluble in methanol (0.5g in 10mL of methanol).

  6. The buffer preparation protocol calls for benzamidine. Should I use the free base or the hydrochloride hydrate salt?

    It is possible that either the benzamidine or the benzamidine hydrochloride hydrate could work for your application. The major difference will be that the benzamidine hydrochloride hydrate (Product No. B6506) is expected to dissolve easier in aqueous solutions. Neither Product 12072 nor the salt Product B5606 is molecular biology, cell culture or biotechnology certified, so their performance once dissolved is likely to be similar.

  7. What is the solution stability of Benzamidine, Product 12072?

    Sigma-Aldrich does not perform formal solution stability testing on this material; however, it is expected to be sensitive to oxidation. It is recommended to prepare solutions fresh for each use.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Purification and characterization of bovine coagulation factor XII (Hageman factor).
H Claeys et al.
European journal of biochemistry, 87(1), 69-74 (1978-06-01)
Hung-Wei Chang et al.
PLoS pathogens, 15(6), e1007826-e1007826 (2019-06-21)
Vaccinia mature virus requires A26 envelope protein to mediate acid-dependent endocytosis into HeLa cells in which we hypothesized that A26 protein functions as an acid-sensitive membrane fusion suppressor. Here, we provide evidence showing that N-terminal domain (aa1-75) of A26 protein
Ana L Roa-Espitia et al.
Biology open, 5(9), 1189-1199 (2016-07-13)
Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function
Use of benzamidine as a proteolytic inhibitor in the radioimmunoassay of glucagon in plasma.
J W Ensinck et al.
The Journal of clinical endocrinology and metabolism, 35(3), 463-467 (1972-09-01)
Fatma Rmili et al.
Biotechnology progress, 35(4), e2833-e2833 (2019-05-03)
A mesophilic bacterial culture, producing an extracellular alkaline lipase, was isolated from the gas-washing wastewaters generated from the Sfax phosphate plant of the Tunisian Chemical Group and identified as Staphylococcus capitis strain. The lipase, named S. capitis lipase (SCL), has

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