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Merck

PC712

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb

liquid, Calbiochem®

Sinónimos:

Anti-53BP1

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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, human
Application:
Citations:
17
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biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:3000-1:5000)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

General description

Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).

Immunogen

Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence

Application

Immunoblotting (1:3000-1:5000)

Immunofluorescence (1:300-1:500, indirect)

Immunoprecipitation (1:300-1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)

Other Notes

Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mariana Acevedo et al.
Cancer research, 76(11), 3252-3264 (2016-05-22)
Promyelocytic leukemia (PML) plays a tumor suppressive role by inducing cellular senescence in response to oncogenic stress. However, tumor cell lines fail to engage in complete senescence upon PML activation. In this study, we investigated the mechanisms underlying resistance to
Hiroshi Ikeuchi et al.
Oncogene (2024-08-03)
Identifying the mechanisms of action of anticancer drugs is an important step in the development of new drugs. In this study, we established a comprehensive screening platform consisting of 68 oncogenes (MANO panel), encompassing 243 genetic variants, to identify predictive
Mafuka Suzuki et al.
PloS one, 18(1), e0281168-e0281168 (2023-01-28)
Malignancy is often associated with therapeutic resistance and metastasis, usually arising after therapeutic treatment. These include radio- and chemo-therapies, which cause cancer cell death by inducing DNA double strand breaks (DSBs). However, it is still unclear how resistance to these