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Merck

A8097

Acridine Orange hydrochloride solution

>95.0% purity (HPLC), solution

Sinónimos:

3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Fórmula empírica (notación de Hill):
C17H19N3 · HCl
Número CAS:
Peso molecular:
301.81
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
3575587

Nombre del producto

Acridine Orange hydrochloride solution, 10 mg/mL in H2O, ≥95.0% (HPLC)

InChI

1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI key

VSTHNGLPHBTRMB-UHFFFAOYSA-N

assay

≥95.0% (HPLC)

form

solution

concentration

10 mg/mL in H2O

technique(s)

microbe id | staining: suitable

color

orange to red

εmax

47-54 at 486-492 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Muta. 2

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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