4-Fluoro-7-nitrobenzofurazan

Name: 4-Fluoro-7-nitrobenzofurazan
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Sinónimos:

NBD-F

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About This Item

Fórmula empírica (notación de Hill):

C₆H₂FN₃O₃

Número CAS:

29270-56-2

Peso molecular:

183.10

UNSPSC Code:

12352100

NACRES:

NA.32

PubChem Substance ID:

329757296

MDL number:

MFCD00010196

Beilstein/REAXYS Number:

1077571

Nombre del producto

4-Fluoro-7-nitrobenzofurazan, BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

product line

BioReagent

assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λ_ex 470 nm; λ_em 528 nm in ethanol
λ_ex 470 nm; λ_em 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

200

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Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.

Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

GHS07

signalword

Warning

hcodes

H315,H319

pcodes

P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

235.4 °F - closed cup

flash_point_c

113 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number

BCCF9196

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Amino acids analysis using a monolithic silica column after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F).

Yanting Song et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)

A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm

4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorogenic labeling reagent for the in vivo analysis of amino acid neurotransmitters using online microdialysis-capillary electrophoresis.

Chanda Ciriacks Klinker et al.

Analytical chemistry, 79(22), 8747-8754 (2007-10-13)

4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for

Fast electrophoretic separation optimization using gradient micro free-flow electrophoresis.

Bryan R Fonslow et al.

Analytical chemistry, 80(9), 3182-3189 (2008-03-21)

The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants.

Fluorescent detection of peptides and amino acids for capillary electrophoresis via on-line derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole.

Hongyi Zhang et al.

Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)

An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample

Simultaneous determination of D-aspartic acid and D-glutamic acid in rat tissues and physiological fluids using a multi-loop two-dimensional HPLC procedure.

Hai Han et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3196-3202 (2011-03-05)

For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and

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