HEPES

Name: HEPES
Brand: SIGMA

≥99.5% (titration), powder, for molecular biology, BioUltra

Sinónimos:

Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)

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About This Item

Fórmula empírica (notación de Hill):

C₈H₁₈N₂O₄S

Número CAS:

7365-45-9

Peso molecular:

238.30

NACRES:

NA.25

PubChem Substance ID:

57651473

UNSPSC Code:

12161700

EC Number:

230-907-9

MDL number:

MFCD00006158

Beilstein/REAXYS Number:

883043

Nombre del producto

HEPES, BioUltra, Molecular Biology, ≥99.5% (T)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (T)

form

powder

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.1% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg
sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.045
λ: 280 nm A_max: 0.035

Quality Level

200

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Tampón HEPES

Application

HEPES has been used as a component of:

DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
Recapture solution to re-capture RNA exosomes from the glycerol gradient
ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Easily compare specifications for HEPES products with the HEPES specification table.

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.

Zhang F, et al.

Nature Protocols, 5(3), 439-439 (2010)

The correction of the metabolic parameters of msg-induced obesity in rats by 2-[4-(benzyloxy) phenoxy] acetic acid.

Konopelniuk V, et al.

Journal of Nutrition and Intermediary Metabolism, 13, 1-9 (2018)

Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells.

Domanski M and LaCava J

Bio-protocol, 7(8) (2017)

Sample Preparation of Telomerase Subunits for Crystallization.

Huang J, et al.

Bio-protocol, 5(16) (2015)

RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells.