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NACRES:
NA.52
UNSPSC Code:
12352200
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Molecular Biology
product line
BioReagent
form
liquid
concentration
200 mM
impurities
oxovanadium V
suitability
in accordance for ribonuclease inhibition test
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.
Application
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes solution has been used:
- as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer
- as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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P A Takizawa et al.
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In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of
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Peritz T, et al.
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