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Merck

94742

Vanadyl ribonucleoside complexes solution

BioReagent, Molecular Biology, 200 mM

Sinónimos:

Ribonucleoside vanadyl complexes

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NACRES:
NA.52
UNSPSC Code:
12352200
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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

liquid

concentration

200 mM

impurities

oxovanadium V

suitability

in accordance for ribonuclease inhibition test

shipped in

dry ice

storage temp.

−20°C

General description

Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.

Application

Vanadyl ribonucleoside complexes solution has been used:
  • as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer
  • as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.


Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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