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Número CAS:
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Specific activity:
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
Biological source:
human liver
Servicio técnico
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Permítanos ayudarlebiological source
human liver
form
buffered aqueous solution
specific activity
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CTSB(1508)
General description
Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.
Application
Cathepsin B from human liver has been used:
- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
Biochem/physiol Actions
Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.
Packaging
Package size based on protein content
Physical form
Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.
Other Notes
One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
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Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts
Roberg K, et al.
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