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Acerca de este artículo
Conjugate:
unconjugated
Clone:
polyclonal
Application:
FACS, IHC, WB
Citations:
10
Servicio técnico
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Permítanos ayudarlebiological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 46-48 kDa
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
flow cytometry: 1:200-1:500, immunohistochemistry: 1:100-1:200, western blot: 1:500-1:1,000
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CNP(1267)
mouse ... Cnp(12799)
rat ... Cnp(25275)
Immunogen
synthetic peptide corresponding to residues 122-136 of human CNPase. The sequence is 93% identical in mouse and rat.
Application
Anti-CNPase antibody produced in rabbit is suitable for flow cytometry at a working dilution of 1:200-1:500, immunohistochemistry at 1:100-1:200 and western blotting at 1:500-1:1000. It was used at a working dilution of 1:1000 as a primary antibody in western blotting of cerebrospinal fluid samples in a study.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. It is expressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes and may be used as a marker of cell differentiation in the CNS.
Physical form
solution in phosphate buffered saline, containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Contenido relacionado
Instructions
Irum Naureen et al.
Fluids and barriers of the CNS, 10(1), 34-34 (2013-12-20)
In hydrocephalus an imbalance between production and absorption of cerebrospinal fluid (CSF) results in fluid accumulation, compression and stretching of the brain parenchyma. In addition, changes in CSF composition have a profound influence on the development and function of the
T Ishii et al.
Neuroscience letters, 268(3), 131-134 (1999-07-16)
We have previously reported that human amniotic epithelial (HAE) cells expressed neuronal and glial cell markers using immunostaining and western blotting. To study the expression system of these cell markers in HAE cells, we investigated the expression of mRNA for
A J Douglas et al.
Annals of human genetics, 56(Pt 3), 243-254 (1992-07-01)
Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI