DUO92104
Duolink® In Situ Orange Starter Kit Mouse/Goat
Sinónimos:
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Kit
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NACRES:
NA.32
UNSPSC Code:
41116158
Fluorescence:
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
Technique(s):
proximity ligation assay: suitable
Servicio técnico
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Duolink®
Quality Segment
technique(s)
proximity ligation assay: suitable
fluorescence
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
suitability
suitable for fluorescence
Application
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
signalword
Danger
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
10 - Combustible liquids
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