E8008
Ethylenediaminetetraacetic acid solution
0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sinónimos:
EDTA solution
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Fórmula empírica (notación de Hill):
C10H16N2O8
Número CAS:
Peso molecular:
292.24
NACRES:
NA.75
PubChem Substance ID:
eCl@ss:
39030907
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
1716295
Servicio técnico
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Permítanos ayudarlesterility
sterile-filtered
Quality Segment
product line
BioReagent
form
solution
concentration
0.02% in DPBS (0.5 mM)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.1-7.7
shipped in
wet ice
storage temp.
2-8°C
SMILES string
OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key
KCXVZYZYPLLWCC-UHFFFAOYSA-N
General description
Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.
Application
Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.
Physical form
Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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