MILLIPLEX® Multiplex Assays Using Luminex®

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Featured Customer Story
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/Mukesh-150px.jpgInterested in how MILLIPLEX® MAP assays can further Zika research?

The MILLIPLEX® MAP Rat Cytokine Panel (Cat. No. RECYTMAG-65K) allowed Dr. Mukesh Kumar, of the University of Hawaii, to develop a guinea pig model for Zika virus infection. Learn more about his research by viewing the video below, and read his most recent publication.

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Need Tips on Optimizing your Multiplex Assays?
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/Sonali-Nayak-headshot.jpgRead the SelectScience Interview with Sonali Nayak, Bioscience Technical Services Manager, Merck:
Discover How Multiplex Assays are Advancing Biomedical Research: Top tips to optimize your analyses 

Get Sonali Nayak’s take on how to best run your MILLIPLEX® MAP assays.

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As your research uncovers the significant role of more and more biomarkers in both normal and disease states, you need a reliable partner for quality assays and consistent performance. We not only offer you the largest portfolio of biomarker assays—including ELISAs, RIAs and MILLIPLEX® MAP (multi-analyte profiling), our kits and reagents provide you with consistent, high-quality tools so that you can do your best work while saving time, labor and cost.

In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our validation process: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® MAP kit includes:
  • Quality controls (QCs) provided to qualify assay performance
  • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
  • Optimized serum matrix to mimic native analyte environment
  • Detection antibody cocktails designed to yield consistent analyte profiles within panel

How to Decide Which MILLIPLEX® Assay Is Best for Your Research

In Method Comparison tests (comparing ELISA and MILLIPLEX® MAP  assays), while the absolute values are not exactly the same, the results do correlate. Hence when switching from one assay method to another, a correction factor may be used when comparing with past data. In most situations the dynamic range and the sensitivity of the assay may be better with the MILLIPLEX® kits.

Please contact Technical Support for more information on correlation factors.

To locate protocols and technical documents for a specific panel, choose any of these options:
  • Search the website for the catalog number.

  • The link to the protocol can be found using:
    • The “Documents” tab
    • The right-hand side of the product name and catalog number
    • The top of the Product Description page
  • To find a panel that contains the analytes you want to measure, use the Analyte Kit Finder at the bottom of this page. Alternatively, type the name of the analyte into the search bar at and use the filters at left to refine your search for “Multiplexing.”

How to Find More Technical Information About Each MILLIPLEX® Assay

  • To find publications citing a specific panel or analyte:
    • Search the website for the catalog number. Links to some of the references using the product can be found at the top of the Product Description Page
    • For a more complete list, contact technical support
  • To determine cross-reactivity for other species for a panel or analyte see the Species Cross-reactivity Tables in our technical guide
  • For intracellular assay kits, we analytically validate the assay only with human cell/tissue culture samples. However, we provide the species homology for each analyte in a table on the product detail page on our website. We have also compiled a list of this information on our website:

    Kits - Kit Species Cross Reactivity

    MAPmate™ Species Cross Reactivity Table

Request the Analyte Quarterly brochure for a complete, updated list of assays.

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カスタマイズ可能なパネルとプレミックスキット – または細胞シグナル伝達MAPmatesアッセイを選択

自分用のMILLIPLEX MAPキットを設計して、価格を確認しましょう。


MILLIPLEX MAPの品質について研究者たちはどのように語っているでしょうか?

メルクミリポアのMILLIPLEX MAPキットを4年間使っています。このキットを使って一連の特異的マーカーを複数分析するところから始めましたが、その信頼性、一貫性と使いやすさから、従来のELISAからすべての実験を移しました。優れた製品であるだけでなく、メルクミリポアの顧客サポートとテクニカルサポートは業界トップクラスです。優れた製品と親身なサポートにより、私の研究能力を伸ばしてくれたので、新しい研究課題に答えることができるようになりました。MILLIPLEX MAPキットは私の研究ツールボックスの重要な要素だと考えています。 

Brian McFarlin, PhD, FACSM(North Texas大学、テキサス州デントン)