数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

DR1025 Sigma-AldrichPhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb

This PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).

PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, Mk, M, R	Rb	Polyclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
DR1025-50ULCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	50 ul	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷ in UV-treated COS cells.
Catalogue Number	DR1025
Brand Family	Calbiochem®
Application Data	Detection of human and monkey Chk1, phosphorylated at Ser³¹⁷, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.

References
References	Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129. Shieh, S.Y., et al. 2000. Genes Dev. 14, 289. Martinho, R.G., et al. 1998. EMBO J. 17, 7239. Zeng, Y., et al. 1998. Nature 395, 507.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive control	Lysates from COS cells treated with UV
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	Immunoblotting (1:1000) Immunocytochemistry (1:500, fluorescence) Paraffin Sections (1:50)
Application Comments	Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Human Monkey Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Do not aliquot.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
DR1025-50ULCN	04055977225914

Documentation

PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb 試験成績書（CoA）

タイトル	ロット番号
DR1025	ロット番号を入力

参考資料

参考資料の概要
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129. Shieh, S.Y., et al. 2000. Genes Dev. 14, 289. Martinho, R.G., et al. 1998. EMBO J. 17, 7239. Zeng, Y., et al. 1998. Nature 395, 507.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	23-August-2007 RFH
Application	Immunoblotting (1:1000) Immunocytochemistry (1:500, fluorescence) Paraffin Sections (1:50)
Application Data	Detection of human and monkey Chk1, phosphorylated at Ser³¹⁷, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
Description	Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.
Background	Chk1 is a downstream protein kinase of ATM/ATR that plays an improtant role in DNA damage checkpoint, development, and tumor suppression. Chk1 is activated in response to various forms of genotoxic stress and replication blocks. Chk1 is activated by phosphorylation on Ser³¹⁷ and Ser³⁴⁵. Chk1 can inactivate cdc25c and phosphorylate p53.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
Isotype	IgG
Species	human, monkey, mouse, rat
Positive control	Lysates from COS cells treated with UV
Form	Liquid
Formulation	In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preservative	None
Comments	Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Do not aliquot.
Toxicity	Standard Handling
References	Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129. Shieh, S.Y., et al. 2000. Genes Dev. 14, 289. Martinho, R.G., et al. 1998. EMBO J. 17, 7239. Zeng, Y., et al. 1998. Nature 395, 507.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

製品がカートに追加されました