MABT1503-100UG | Anti-PGM5 (Aciculin) Antibody, clone 14F8

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100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Rb, M, R, H ELISA, IF, IHC, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT1503-100UG
      DescriptionAnti-PGM5 (Aciculin) Antibody, clone 14F8
      Alternate Names
      • Aciculin
      • Phosphoglucomutase-like Protein 5
      • PGM-RP
      Background InformationPhosphoglucomutase-like protein 5 (UniProt: Q15124; also known as Aciculin, Phosphoglucomutase-related protein, PGM-RP) is encoded by the PGM5 (also known as PGMRP) gene (Gene ID: 5239) in human. Aciculin is a dystrophin and utrophin-binding protein that is highly homologous to the glycolytic enzyme phosphoglucomutase type 1. However, it lacks phosphoglucomutase activity. Aciculin is a component of adherens-type cell-cell and cell-matrix junctions and is concentrated in focal contacts at the ends of actin bundles and associates with actin filaments. Aciculin is also reported to be an interaction partner of Filamin C and Xin proteins. Higher levels of Aciculin are reported in smooth and cardiac muscle and lower levels are present in skeletal muscle, liver, kidney and brain tissue. In striated muscle, it mainly localizes to the intercalated discs of the heart and to myotendinous junctions. Low levels of Aciculin are expressed in 10-12-week-old fetus and these levels increase progressively into infancy reaching maximum levels at maturity. Two isoforms of Aciculin have been reported that are produced by alternative splicing. Aciculin knockdown in myotubes is shown to cause failure in myofibril assembly, alignment and membrane attachment, and a massive reduction in myofibril number. (Ref.: Molt, S., et al., (2014). J. Cell Sci.127(16); 3578-3592).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PGM5 (Aciculin), clone 14F8, Cat. No. MABT1503, is a mouse monoclonal antibody that detects Phosphoglucomutase-like protein 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected PGM5 (Aciculin) in rat heart tissue lysate.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated PGM5 (Aciculin) in Immunoprecipitation applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

      Immunofluorescence Analysis: A representative lot detected PGM5 (Aciculin) in Immunofluorescence applications (Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

      Immunohistochemistry Analysis: A representative lot detected PGM5 (Aciculin) in Immunohistochemistry applications (Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

      Western Blotting Analysis: A representative lot detected PGM5 (Aciculin) in Western Blotting applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

      ELISA Analysis: A representative lot detected PGM5 (Aciculin) in ELISA applications (Molt, S., et. al. (2014). J Cell Sci. 127(Pt 16):3578-92).
      Biological Information
      ImmunogenCytoskeletal fraction of the human myometrium tissue extract.
      Clone14F8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 14F8 detects Phosphoglucomutase-like protein 5 in mouse and rat cardiac tissue and is predicted to react with human.
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Rabbit
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteMouse, Rat, Bovine, Human, Rabbit. Predicted to react with Porcine, Avian, and Frog.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PGM5
      • PGMRP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~57 kDa observed; 62.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse heart tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected PGM5 (Aciculin) in mouse heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications