MABT867 | Anti-Podoplanin Antibody, clone LpMab-17

新着MABT867
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT867
      DescriptionAnti-Podoplanin Antibody, clone LpMab-17
      Alternate Names
      • Aggrus
      • Glycoprotein 36
      • Gp36
      • PA2.26 antigen
      • T1-alpha
      • T1A
      Background InformationPodoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-17 is reported to bind to non-PLAG domain and detects endogenous podoplanin in cancer cells and normal cells. It recognizes glycan-deficient podoplanin indicating that its interaction with podoplanin is independent of its glycosylation. The minimum epitope of LpMab-17 is identified as Gly77-Asp82. Although human and monkey podoplanin exhibit 94% homology, this clone does not recognize monkey podoplanin. (Ref.: Kato, Y., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(2); 109-116).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Podoplanin, clone LpMab-17, Cat. No. MABT867, is a mouse monoclonal antibody that detects human podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.

      Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

      Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

      Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).

      ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(2):109-16).
      Biological Information
      ImmunogenLN229 glioma cells expressing human Podoplanin.
      CloneLpMab-17
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone LpMab-17 detects podoplanin in human cells. It detects PLAG4 domain. The minimal epitope seunce is shown to be Gly77-Asp82.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDPN
      • GP36
      • PSEC0003
      • PSEC0025
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight16.7 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human colon cancer tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human colon cancer tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications