MABT851 Anti-Podoplanin Antibody, clone PMab-1

MABT851
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB, IH(P), ICC, FC R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT851
      DescriptionAnti-Podoplanin Antibody, clone PMab-1
      Alternate Names
      • Aggrus
      • Glycoprotein 38
      • Gp38
      • OTS-8
      • PA2.26 antigen
      • T1-alpha
      • T1A
      • Transmembrane glycoprotein E11
      Background InformationPodoplanin (UniProt: Q62011; also known as Aggrus, Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 14726) in murine species. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts. High expression of Podoplanin is observed in colon carcinomas. . It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside. Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models. It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing. (Ref.: Maruyama, Y et al (2014). Invest.Ophthal. Visual Sci. 55(8); 4813-4822).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Podoplanin, clone PMab-1, Cat. No. MABT851, is a rat monoclonal antibody that detects mouse Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in mouse lung tissue.

      Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem. 45(4):227-37).

      Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

      Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

      Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem. 45(4):227-37).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Podoplanin.
      ClonePMab-1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone PMab-1 detects murine Podoplanin. It targets an epitipe within 14 amino acids from the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pdpn
      • Gp38
      • Ots8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed; 18.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse colon tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Podoplanin in 10 µg of mouse colon tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications