MABT850 Anti-Podoplanin Antibody, clone PMab-2

MABT850
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R ELISA, FC, IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT850
      DescriptionAnti-Podoplanin Antibody, clone PMab-2
      Alternate Names
      • E11 antigen epitope
      • Glycoprotein 38
      • Gp38
      • OTS-8
      • RTI140
      • T1-alpha
      • T1A
      • Type I cell 40 kDa protein
      Background InformationPodoplanin (UniProt: Q64294; also known as E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein) is encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 54320) in rat. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-135), a helical domain (aa 136-156) and a cytoplasmic region (aa 157-166). Podoplanin is extensively O-glycosylated and contains sialic acid residues. O-glycosylation is believed to be essential for its platelet aggregation activity. Clone PMab2 exhibits high affinity for podoplanin (Kd = 0.59 nM). (Ref.: Oki, H et al. (2015). Monoclon. Antib. Immunodiagn. Immunother. 34(6); 396-403).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Podoplanin, clone PMab-2, Cat. No. MABT850, is a mouse monoclonal that detects rat podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in rat spleen tissue.

      Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

      Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

      Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

      ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of rat Podopalnin.
      ClonePMab-2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PMab-2 detects podoplanin in rat spleen and kidney. It targets an epitope within 14 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Pdpn
      • Gp38
      • Ots8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight17.58 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat kidney tissue sections.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in rat kidney tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications