MABC1137-25UG Anti-RKIP Antibody, clone 4A11

新着MABC1137-25UG
25 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC1137-25UG
      DescriptionAnti-RKIP Antibody, clone 4A11
      Alternate Names
      • Raf kinase inhibitor protein
      • Phosphatidylethanolamine-binding protein 1
      • PEBP-1
      • HCNPpp
      • Neuropolypeptide h3
      • Prostatic-binding protein
      Background InformationPhosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 4A11 exhibits specific staining in formalin-fixed NIH3T3 cells transfected with GFP-PEBP1 and in some pancreatic cancer cells. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibodyIgG1κ in buffer containing PBS without azide
      Applications
      ApplicationDetects Raf kinase inhibitor protein (Phosphatidylethanolamine-binding protein 1) using this mouse monoclonal Anti-RKIP, clone 4A11, Cat. No. MABC1137, tested for use in Western Blotting, Immunocytochemistry, and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RKIP in human pancreas and human thyroid tissue sections.

      Immunohistochemistry Analysis: A representative lot of this antibody detected RKIP in Immunohistochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).

      Immunocytochemistry Analysis: A representative lot of this antibody detected RKIP in Immunocytochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
      Biological Information
      ImmunogenPurified native and denatured Raf kinase inhibitor protein (RKIP).
      Clone4A11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4A11 specifically detects human Raf kinase inhibitor protein, (RKIP).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PEBP1
      • PBP
      • PEBP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications