MABC1191-100UG | Anti-Sodium-Iodide Symporter Antibody, clone ARN1

新着MABC1191-100UG
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC1191-100UG
      DescriptionAnti-Sodium-Iodide Symporter Antibody, clone ARN1
      Alternate Names
      • Sodium/iodide cotransporter
      • Na(+)/I(-) cotransporter
      • Na(+)/I(-) symporter
      • Solute carrier family 5 member 5
      Background InformationSodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It is found in the basolateral membrane of thyroid follicular cells where it mediates efficient iodide uptake from the bloodstream into the thyroid using the sodium gradient generated by Na+/K+-ATPase. It transports one iodide ion along with two sodium ions following the inwardly directed sodium gradient. Sodium/iodide cotransporter expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Mutations in SLC5A5 gene are known to cause thyroid dyshormonogenesis (TDH1) that is characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 140 mM Tris and 180 mM Glycine (pH 7.4) without azide.
      Applications
      ApplicationAnti-Sodium-Iodide Symporter, clone ARN1, Cat. No. MABC1191, is a mouse monoclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sodium-Iodide Symporter in human stomach tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry applications (Tazebay, U.H., et. al. (2000). 6(8):871-8).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium/iodide cotransporter.
      EpitopeC-terminus
      CloneARN1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone ARN1 is a mouse monoclonal antibody that detects Sodium/iodide cotransporter in human cells. It targets an epitope within 13 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC5A5
      • NIS
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~68 kDa observed; 68.67 kda calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDCK cells transduced with human WT NIS.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Sodium-Iodide Symporter in MDCK cells transduced with human WT Sodium-Iodide Symporter (NIS).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications