MABS1687-100UG | Anti-p70S6 Kinase Antibody, clone 10H11.1

新着MABS1687-100UG
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS1687-100UG
      DescriptionAnti-p70S6 Kinase Antibody, clone 10H11.1
      Alternate Names
      • Ribosomal protein S6 kinase beta-1
      • EC 2.7.11.1
      • S6K-beta-1
      • S6K1
      • 70 kDa ribosomal protein S6 kinase 1
      • P70S6K1
      • p70-S6K 1
      • Ribosomal protein S6 kinase I
      • Serine/threonine-protein kinase 14A
      • p70 ribosomal S6 kinase alpha
      • p70 S6 kinase alpha
      • p70 S6K-alpha
      • p70 S6KA
      Background InformationRibosomal protein S6 kinase beta-1 (UniProt: P23443; also known as EC 2.7.11.1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA) is encoded by the RPS6KB1 (also known as STK14A) gene (Gene ID: 6198) in human. P70S6K1 is a serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth, and cell cycle progression. It regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Upon mitogenic stimulation, phosphorylation by mTORC1 leads to its activation following dissociation from the EIF3 complex. The active p70S6K1 then phosphorylates and activates several substrates in the pre-initiation complex. It is also shown to play a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, which results in the inhibition of mTORC2 and Akt1 signaling. The protein kinase domain of p70S6K1 is localized to amino acids 910352 and the autoinhibitory domain is localized to amino acids 424-525. The autoinhibitory domain blocks phosphorylation within the AGC-kinase C-terminal domain and the activation loop. It also contains a TOR signaling (TOS) motif (aa 28-32) that is essential for its activation by mTORC1. Five isoforms of p70S6K1 have been described that are produced by alternative splicing and alternative initiation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-p70S6 Kinase, clone 10H11.1, Cat. No. MABS1687, is a mouse monoclonal antibody that detects Ribosomal protein S6 kinase beta-1 and has ben tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected p70S6 Kinase in human kidney and human liver tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to the first 93 amino acids from the N-terminal region of human Ribosomal protein S6 kinase beta-1.
      Clone10H11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10H11.1 is a mouse monoclonal antibody that detects human Ribosomal protein S6 kinase beta-1. It targets an epitope within the first 93 amino acids from the N-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPS6KB1
      • STK14A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed; 59.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected p70S6 Kinase in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications