07-760-I | Anti-phospho-eIF-2α (Ser51)

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      Replacement Information
      Catalogue Number07-760-I
      Brand Family Upstate
      DescriptionAnti-phospho-eIF-2α (Ser51)
      Alternate Names
      • Eukaryotic translation initiation factor 2 subunit
      • Eukaryotic translation initiation factor 2 subunit alpha
      • eIF-2-alpha
      Background InformationNuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-phospho-eIF-2 (Ser51), Cat. No. 07-760-I, is a rabbit polyclonal antibody that detects Eukaryotic translation initiation factor 2 subunit 1 and has been tested for use in Peptide Inhibition Assay and Western Blotting.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesPeptide Inhibition Assay: A 1:1,000 dilution from a representative lot detected phospho-eIF-2 (Ser51) in HeLa treated Calyculin A.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphorylated ser51 of human Eukaryotic translation initiation factor 2 subunit 1.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis rabbit polyclonal antibody detects elf-2 alpha phosphorylated on Serine 51 in human.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Drosophila, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • eIF-2alpha
      • eIF2a
      • CG9946
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~38 kDa observed; 38.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa treated with Calyculin A cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eIF-2 (Ser51) in HeLa treated with Calyculin A cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information