D6883
2′,7′-Dichlorodihydrofluorescein diacetate
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
Synonyme(s) :
2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate
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A propos de cet article
Formule empirique (notation de Hill) :
C24H16Cl2O7
Numéro CAS:
Poids moléculaire :
487.29
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
4610273
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2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
Quality Level
assay
≥97%
form
powder
storage temp.
−20°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
Catégories apparentées
General description
2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
Application
2′,7′-Dichlorofluorescin diacetate is suitable for use in:
- sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
- microplate assay for detecting oxidative products in phagocytic cells
- quantitative multiwell myeloid differentiation assay
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
Preparation Note
This product should be stored in powdered format at -20°C.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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