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Merck

IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

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Product Name

Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

Quality Level

400

Related Categories

General description

Immobilon-P transfer membrane is recommended for most Western blotting applications, especially proteins greater than 20 kDa. Immobilon-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Features and Benefits

Immobilon-P PVDF Membrane:
  • Won′t crack, curl or fracture when cut
  • Low background
  • Superior staining capabilities
  • Can be reprobed multiple times

Other Notes

Filter Code: IPVH
Primary Binding Mechanisms: Electrostatic, hydrophobic
Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isabelle Robert et al.
The Journal of experimental medicine, 206(5), 1047-1056 (2009-04-15)
Immunoglobulin class switch recombination (CSR) is initiated by DNA breaks triggered by activation-induced cytidine deaminase (AID). These breaks activate DNA damage response proteins to promote appropriate repair and long-range recombination. Aberrant processing of these breaks, however, results in decreased CSR
Pulmonary artery smooth muscle cell senescence is a pathogenic mechanism for pulmonary hypertension in chronic lung disease.
Noureddine, Hibo, et al.
Circulation Research, 109, 543-553 (2011)
Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor.
Dwyer, J, et al.
Oncogene, 30, 190-200 (2011)
Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.
Bourg, Gisele, et al.
Journal of Bacteriology, 191, 2985-2992 (2009)
Hao Zhang et al.
Journal of animal science and biotechnology, 11, 52-52 (2020-06-10)
Evidence indicates that early weaning predisposes piglets to intestinal oxidative stress and increases the risk of intestinal dysfunction; however, there are minimal satisfactory treatment strategies for these conditions. This study investigated the potential of resveratrol and its analog, pterostilbene, as
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Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKUGTIN
IPVH0001008436037122422

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