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Merck

GF156

B18R, Human Recombinant Carrier-Free

liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)

Synonyme(s) :

B18R protein, Recombinant B18R

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160405

Nom du produit

B18R, Human Recombinant Carrier-Free, liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)

biological source

human

assay

>98% (SDS-PAGE)

form

liquid

specific activity

≥1.3 x 10e6 units/mg protein

mol wt

Mw 38,389 Da

concentration

0.5 mg/mL

technique(s)

cell culture | stem cell: suitable

impurities

<0.05 ng/μg cytokine (LAL test)

input

sample type induced pluripotent stem cell(s)

solubility

water: soluble

suitability

suitable for molecular biology

shipped in

dry ice

Quality Level

100

Analysis Note

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

Application

B18R, Human RecombinantCarrier-Free has been used as a decoy receptor to study the intrinsic antiviralactivity of recombinant soluble IFNα/β cell surface receptor 2 (sIFNAR2). Ithas also been used in the generation and maintenance of induced pluripotentstem cells (iPSCs) and human IPSCs.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

Physical form

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

Preparation Note

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Irene L Llorente et al.
Stem cell research, 55, 102458-102458 (2021-07-19)
White matter stroke (WMS) occurs as small infarcts in deep penetrating blood vessels in the brain and affects the regions of the brain that carry connections, termed the subcortical white matter. WMS progresses over years and has devastating clinical consequences.
Isaac Hurtado-Guerrero et al.
Journal of clinical medicine, 9(4) (2020-04-05)
Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß) receptor is generated through alternative splicing of IFNAR2 and
Roni A Hazim et al.
Stem cell research & therapy, 8(1), 217-217 (2017-10-04)
Dysfunction of the retinal pigment epithelium (RPE) is implicated in numerous forms of retinal degeneration. The readily accessible environment of the eye makes it particularly suitable for the transplantation of RPE cells, which can now be derived from autologous induced

Numéro d'article de commerce international

RéférenceGTIN
GF15604055977116397

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