B6429
Casein Blocking Buffer 10x
for Northern and Southern blotting, solution
Synonyme(s) :
10x Blocking Buffer
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NACRES:
NA.54
UNSPSC Code:
12161700
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Molecular Biology
Quality Level
form
solution
technique(s)
Southern blotting: suitable
storage temp.
room temp
General description
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Application
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Features and Benefits
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
Preparation Note
Dilute 10x Blocking Buffer with molecular biology grade water before use.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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