E5036

Epidermal Growth Factor Protein, human

Name: Epidermal Growth Factor Protein, human
Brand: SIGMA

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

EGF

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About This Item

Numéro CAS:

62253-63-8

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00132565

Nom du produit

Facteur de croissance épidermique human, EGF, recombinant, expressed in Escherichia coli, >97% (SDS-PAGE)

SMILES string

S(CC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]%12N(CCC%12)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N

InChI

1S/C270H401N73O83S7/c1-24-134(19)217(262(420)293-112-201(355)298-161(69-74-205(359)360)229(387)301-160(45-35-82-286-269(279)280)228(386)333-192(119-428)255(413)307-162(68-73-198(274)352)230(388)316-176(94-141-54-64-150(350)65-55-141)241(399)304-159(44-34-

InChI key

GVUGOAYIVIDWIO-UFWWTJHBSA-N

biological source

human

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.08-0.8 ng/mL EC₅₀

mol wt

~6 kDa

packaging

pkg of 200 and 500 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

≤1 EU/μg Endotoxin

color

white

solubility

water: soluble 0.190-0.210, clear, colorless

UniProt accession no.

P01133

storage temp.

−20°C

Quality Level

200

Gene Information

human ... EGF(1950)

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Application

Epidermal Growth Factor, human, animal component free has been used:

as a supplement in in LHC-8 medium to culture liver cell lines
in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells
as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cells
as a component in tumorsphere medium

Biochem/physiol Actions

Epidermal growth factor (EGF) helps to induce cell growth, proliferation and differentiation. It participates in the repairing of renal tissues in the kidney. It promotes the reabsorption of magnesium with the help of the transient receptor potential cation channel 6 (TRMP6). EGF is known to participate in the pathophysiology of drug-induced renal magnesium loss.

General description

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.^† Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.^† EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.^† EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,^† which appears to utilize the EGF receptor to gain entry into cell† EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.^†

Epidermal growth factor (EGF) is synthesized in Henle′s loop and the distal convoluted tubule in the kidney. It is also produced in salivary glands and duodenum.

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Cancer-associated fibroblasts induce epithelial-mesenchymal transition through secreted cytokines in endometrial cancer cells

Wang X, et al.

Oncology Letters, 15(4), 5694-5702 (2018)

Epidermal growth factor and its influencing variables in healthy children and adults

Meybosch S, et al.

Testing, 14(1), e0211212-e0211212 (2019)

Gab3 overexpression in human glioma mediates Akt activation and tumor cell proliferation

Jia P, et al.

Testing, 12(3), e0173473-e0173473 (2017)

In vitro tumorsphere formation assays

Johnson S, et al.

Bio-protocol, 3(3), e325-e325 (2013)

DRR regulates AKT activation to drive brain cancer invasion.

A Dudley et al.

Oncogene, 33(41), 4952-4960 (2013-10-22)

Glioblastoma (GBM) is the most common and invasive adult brain cancer. The rapid invasion of cancer cells into the normal brain is a major cause of treatment failure, yet the mechanisms that regulate this process are poorly understood. We have

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Data Sheet - E5036

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