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Merck

P4932

Polymyxine B sulfate salt

powder, non-animal origin, suitable for cell culture, BioReagent

Synonyme(s) :

Polymixin, Polymixin B, Polymixin B sulfate

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About This Item

Formule empirique (notation de Hill) :
C55H96N16O13 · 2H2SO4
Numéro CAS:
1405-20-5
Poids moléculaire :
1385.61
NACRES:
NA.76
UNSPSC Code:
12352207
EC Number:
215-774-7
MDL number:
MFCD00131991

Nom du produit

Polymyxine B sulfate salt, powder, BioReagent, suitable for cell culture

InChI key

HNDFYNOVSOOGDU-UHFFFAOYSA-N

InChI

1S/C48H82N16O13.H2O4S/c1-27(2)24-37-47(76)59-32(11-19-52)41(70)56-31(10-18-51)43(72)61-35(14-22-65)39(68)54-21-13-34(45(74)57-33(12-20-53)44(73)64-38(48(77)63-37)25-28-6-4-3-5-7-28)60-42(71)30(9-17-50)58-46(75)36(15-23-66)62-40(69)29(8-16-49)55-26-67;1-5(2,3)4/h3-7,26-27,29-38,65-66H,8-25,49-53H2,1-2H3,(H,54,68)(H,55,67)(H,56,70)(H,57,74)(H,58,75)(H,59,76)(H,60,71)(H,61,72)(H,62,69)(H,63,77)(H,64,73);(H2,1,2,3,4)

SMILES string

O=C(C(NC(C(CCN)NC(CCCCC(C)CC)=O)=O)C(C)O)NC(CCN)C(NC(CCNC(C(NC(C(NC(C(CCN)N1)=O)CCN)=O)C(O)C)=O)C(NC(CCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C1=O)=O)=O)=O)=O.O=S(O)(O)=O

product line

BioReagent

form

powder

potency

≥6,000 USP units per mg

technique(s)

cell culture | mammalian: suitable

antibiotic activity spectrum

Gram-negative bacteria

mode of action

cell membrane | interferes

storage temp.

2-8°C

Quality Level

200

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Catégories apparentées

Biochem/physiol Actions

Mode d'action : Le sulfate de polymyxine B se lie à la partie lipide A des lipopolysaccharides bactériens†, ce qui altère la membrane cytoplasmique en induisant la formation de pores suffisamment grands pour provoquer une fuite de nucléotides dans les parois bactériennes. Ceci modifie la perméabilité de la membrane cytoplasmique.

Spectre antimicrobien : Présente une action bactéricide vis-à-vis de la plupart des bacilles à Gram négatif††, notamment E. Coli et la plupart des champignons et des bactéries à Gram positif.
Polymyxin B sulfate has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has been used in studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.

Application

Polymyxin B sulfate salt has been used:
  • for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor
  • as positive and negative control during microbicidal concentration (MCC) assay

Disclaimer

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

General description

Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections.
Chemical structure: peptide

Other Notes

1mu,5mu
Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Preparation Note

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000485050
0000485050
0000465846
0000465846
0000421309

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Franz G Zingl et al.
Cell host & microbe, 27(2), 225-237 (2020-01-07)
Gram-negative bacteria release outer membrane vesicles into the external milieu to deliver effector molecules that alter the host and facilitate virulence. Vesicle formation is driven by phospholipid accumulation in the outer membrane and regulated by the phospholipid transporter VacJ/Yrb. We use
P Dello Sbarba et al.
Cytokine, 8(6), 488-494 (1996-06-01)
Macrophage colony-stimulating factor (M-CSF) is the main growth factor for mononuclear phagocytes. Responsiveness to growth factors is reduced in the course of functional activation of macrophages. We studied the interference of the macrophage activator interleukin 2 (IL-2) with the response
J W Kang et al.
Journal of chromatography. A, 879(2), 211-218 (2000-07-13)
A capillary zone electrophoresis method for analysis of polymyxin B sulfate is described. In this method, triethanolamine (TEA)-phosphate buffer at pH 2.5 was employed to reduce the adsorption of analyte onto the capillary wall. Methyl-beta-cyclodextrin (M-beta-CD) and 2-propanol (IPA) were
M C Skinner et al.
Antimicrobial agents and chemotherapy, 53(4), 1482-1489 (2009-02-04)
A topical microbicide that women can use to prevent sexually transmitted diseases (STDs) is essential, and many microbicide candidates are being tested for activity against human immunodeficiency virus and other STDs, including Chlamydia trachomatis. Screening assays for assessing the activity
Sineenart Sengyee et al.
PLoS neglected tropical diseases, 12(2), e0006287-e0006287 (2018-02-24)
Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high
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