On the production of 12 alpha-hydroxysteroid dehydrogenase from Clostridium group P, strain C48-50 ATCC 29733.

The production of 12 alpha-hydroxysteroid dehydrogenase of Clostridium group P strain C48-50 was optimized when the organism was grown in the presence of 2% fructose and 0.1% dithiothreitol. It appears that an initial redox potential of less than -160 mV (achieved by autoclaving in the presence of dithiothreitol, dithioerythritol or cysteine) is important in the production of this enzyme.