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Merck

F6636

Nutrient Mixture F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-12

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

Nombre del producto

Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture

form

powder

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Preparation Note

Formulated to contain 11.5 grams of powder per liter of medium.
Supplement with 2.68 g/L sodium bicarbonate.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000507390
0000507390
25C097
25C097
0000387282

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