NA0200
GenElute™ HP Plasmid Midiprep Kit
sufficient for 25 purifications
Sinónimos:
GenElute™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
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NACRES:
NA.52
UNSPSC Code:
41105501
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sufficient for 25 purifications
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
Quality Level
test parameters
sample volume: 1-5 mL bacterial culture
greener alternative category
, Aligned
storage temp.
15-25°C
Categorías relacionadas
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
Application
GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 350 μg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- No phenol/chloroform extraction or alcohol precipitation required
- Contains fewer plastic components, reducing the amount of waste
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
signalword
Danger
target_organs
Central nervous system
Clase de almacenamiento
3 - Flammable liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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