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AP1048 Anti-PARP10 Rabbit pAb

Anti-PARP10 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Poly(ADP-Ribose) Polymerase pAb

AP1048
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~110 kDa PARP10 protein in U937 cells.
Catalogue NumberAP1048
Brand Family Calbiochem®
SynonymsAnti-Poly(ADP-Ribose) Polymerase pAb
Application Data
Detection of human PARP10 by immunoblotting. Sample: Whole cell lysate (30 µg) from U937 cells. Primary antibody: Anti-PARP10 Rabbit pAb (Cat. No. AP1048) (1 µg/ml). Detection: Chemiluminescence
References
ReferencesChou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
Lesniewicz, K., et al. 2005. Genomics 86, 38.
Yu, M., et al. 2005. Oncogene 24 1982.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlU937 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids in the middle region of human PARP10
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AP1048 0

Documentation

References

Hivatkozások áttekintése
Chou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
Lesniewicz, K., et al. 2005. Genomics 86, 38.
Yu, M., et al. 2005. Oncogene 24 1982.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Poly(ADP-Ribose) Polymerase pAb
ApplicationImmunoblotting (1 µg/ml)
Application Data
Detection of human PARP10 by immunoblotting. Sample: Whole cell lysate (30 µg) from U937 cells. Primary antibody: Anti-PARP10 Rabbit pAb (Cat. No. AP1048) (1 µg/ml). Detection: Chemiluminescence
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~110 kDa PARP10 protein.
BackgroundA novel member of the poly(ADP-ribose) polymerase (PARP) family, PARP10 has been characterized as a novel c-myc-interacting protein. PARP family members regulate transcription, genomic stability, and DNA metabolism, especially in response to DNA damage. PARP10 can autopolyribosylate as well as ribosylate core histones and is found in both the cytoplasm and nucleus. In cells, PARP10 associated with the nucleolus is phosphorylated in a Cdk dependent manner. In vitro Cdk2/cyclin E can regulate PARP10 activity through phosphorylation. Phosphoryated PARP-10 is absent in growth-arrested cells. Overexpression of PARP10 results in loss of cell viability, while down-regulation results in delayed G1 progression and apoptosis. These results suggest that PARP10 functions in cell proliferation and may serve as a marker for proliferating cells.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids in the middle region of human PARP10
IsotypeIgG
Specieshuman
Positive controlU937 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
Lesniewicz, K., et al. 2005. Genomics 86, 38.
Yu, M., et al. 2005. Oncogene 24 1982.