Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DR1104 Anti-SMC3 Rabbit pAb

DR1104
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~150 kDa SMC3 protein.
Catalogue NumberDR1104
Brand Family Calbiochem®
SynonymsAnti-Structural Maintenance of Chromosome 3
Application Data
Detection of human SMC3 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with SMC3. Antibody for IP: Anti-SMC3 Rabbit pAb (Cat. No. DR1104) (ratio of Ab to protein A beads 1:50-1:100); Antibody for IP Anti-SMC3 Mouse pAb. Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationUndiluted serum.
PreservativeNone
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsFor immunoprecipitation the recommended ratio of antibody to protein A beads is 1:50-1:100; the dilution of antibody must be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human SMC3 (aa 1-1217)
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
DR1104 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Structural Maintenance of Chromosome 3
ApplicationImmunoprecipitation (see comments)
Application Data
Detection of human SMC3 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with SMC3. Antibody for IP: Anti-SMC3 Rabbit pAb (Cat. No. DR1104) (ratio of Ab to protein A beads 1:50-1:100); Antibody for IP Anti-SMC3 Mouse pAb. Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~150 kDa SMC3 protein.
BackgroundThis protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation.
HostRabbit
Immunogen speciesHuman
ImmunogenFull-length, human SMC3 (aa 1-1217)
IsotypeIgG
Specieshuman
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsFor immunoprecipitation the recommended ratio of antibody to protein A beads is 1:50-1:100; the dilution of antibody must be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review