234133 Collagenase, Paralithodes camtschatica Hepatopancreas

234133
  
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      Áttekintés

      Replacement Information

      Kulcsspecifikációk táblázata

      Description
      Overview

      This product has been discontinued.



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      Mixture of collagenolytic proteases that can be separated and characterized by FPLC. Has high activity against different types of collagen, especially calf skin collagen Type III and bovine lens capsule collagen Type IV, that are resistant to clostridial collagenases. Excellent, general-purpose protease that has chymotrypsin-, trypsin-, and elastase-like specificity and cleaves both standard synthetic and protein substrates. Ability to cleave native, insoluble collagen makes it particularly suitable for isolation of viable cells from tissues. Recommended for harvesting of endothelial cells.
      Note: 1 KU = 1000 units.
      Catalogue Number234133
      Brand Family Calbiochem®
      References
      ReferencesKlimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
      Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.
      Product Information
      CAS number9001-12-1
      Activity≥1,000 Mandl units/mg dry weight
      Unit of DefinitionOne Mandl unit is defined as the amount of enzyme that will release 1.0 µmol Leu equivalent from collagen in 5 h at 37°C, pH 7.5.
      EC number3.4.24.3
      FormLyophilized
      Hygroscopic Hygroscopic
      PI2.3, temperature optimum 37°C
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42/43

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-24-37

      Do not breathe dust.
      Avoid contact with skin.
      Wear suitable gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Collagenase, Paralithodes camtschatica Hepatopancreas MSDS

      Title

      Safety Data Sheet (SDS) 

      Collagenase, Paralithodes camtschatica Hepatopancreas Certificates of Analysis

      TitleLot Number
      234133

      References

      Hivatkozások áttekintése
      Klimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
      Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-April-2008 RFH
      DescriptionMixture of collagenolytic proteases that can be separated and characterized by FPLC. Excellent general purpose protease with chymotrypsin-, trypsin-, and elastase-like specificity. Cleaves both standard synthetic and protein substrates. Has high activity against different types of collagen, especially calf skin collagen Type III and bovine lens capsule collagen Type IV, that are resistant to clostridial collagenases. Has the ability to cleave native, insoluble collagen, making it particularly suitable for the isolation of intact viable cells from tissues. Recommended for harvesting of endothelial cells. Stable at pH 6.5-8.5, and has an optimal pH and temperature of 7.7 and 37°C, respectively.
      FormLyophilized
      CAS number9001-12-1
      EC number3.4.24.3
      Activity≥1,000 Mandl units/mg dry weight
      Unit definitionOne Mandl unit is defined as the amount of enzyme that will release 1.0 µmol Leu equivalent from collagen in 5 h at 37°C, pH 7.5.
      SolubilityAqueous Buffers (1 mg/ml)
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 2481
      ReferencesKlimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
      Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.