M4159

Methylene Blue hydrate

Name: Methylene Blue hydrate
Brand: SIGMA

suitable for nucleic acid staining, BioReagent

Synonym(s):

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent

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All Photos(3)

About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₈ClN₃S · xH₂O

CAS Number:

122965-43-9

Molecular Weight:

319.85 (anhydrous basis)

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654386

EC Number:

200-515-2

Beilstein/REAXYS Number:

3599847

MDL number:

MFCD00150006

Product Name

Methylene Blue hydrate, suitable for nucleic acid staining, BioReagent

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

grade

Molecular Biology

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL (dark blue)

suitability

suitable for nucleic acid staining

storage temp.

room temp

Quality Level

100

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Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Features and Benefits

Less hazardous than staining with ethidium bromide
Viewable in visible light
Fast and easy to use

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

pictograms

GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Hydrogen bond acceptors and additional cationic charges in methylene blue derivatives: photophysics and antimicrobial efficiency.

Ariane Felgenträger et al.

BioMed research international, 2013, 482167-482167 (2013-03-20)

Photodynamic inactivation of bacteria (PIB) by efficient singlet oxygen photosensitizers might be a beneficial alternative to antibiotics in the struggle against multiresistant bacteria. Phenothiazinium dyes belong to the most prominent classes of such sensitizers due to their intense absorption in

Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

A C Peacock et al.

Biochemistry, 6(6), 1818-1827 (1967-06-01)

Young-Sharp, D. and Kumar, R.

Technique, 1, 183-183 (1989)

Phenotypic identification of the redox dye methylene blue as an antagonist of heat shock response gene expression in metastatic melanoma cells.

Angela L Davis et al.

International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)

Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of

Increased origin activity in transformed versus normal cells: identification of novel protein players involved in DNA replication and cellular transformation.

Domenic Di Paola et al.

Nucleic acids research, 38(7), 2314-2331 (2010-01-13)

Using libraries of replication origins generated previously, we identified three clones that supported the autonomous replication of their respective plasmids in transformed, but not in normal cells. Assessment of their in vivo replication activity by in situ chromosomal DNA replication

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