Key Documents
AB142
Anti-Galactocerebroside Antibody
serum, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rabbit, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
suitability
not suitable for immunohistochemistry (Paraffin)
shipped in
dry ice
Gene Information
human ... GALC(2581)
Immunogen
Galactocerebroside purified from bovine brain.
Application
Anti-Galactocerebroside Antibody detects level of Galactocerebroside & has been published & validated for use in ELISA, IC, IH.
Immunohistochemistry: suggested starting dilution 1:10-1:50. Not suggested for use on paraffin embedded tissue sections.
Immunocytochemistry: suggested starting dilution 1:10-1:50. For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. Staining of living cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.
The diluent of choice is DMEM but other buffers may be used.
The recommended fixative is 4% paraformaldehyde.
ELISA: suggested starting dilution 1:50-1:100.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: suggested starting dilution 1:10-1:50. For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. Staining of living cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.
The diluent of choice is DMEM but other buffers may be used.
The recommended fixative is 4% paraformaldehyde.
ELISA: suggested starting dilution 1:50-1:100.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Oligodendrocytes in mouse brain culture cells (live). AB142 has also shown staining on fixed mouse brain tissue. Other possible applications are staining of rat and human living brain cells / tissue.
Physical form
Rabbit antisera. Liquid. Contains no preservative.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jae Woong Lee et al.
The Journal of nutrition, 139(10), 1987-1993 (2009-08-07)
Alzheimer's disease (AD) is characterized by the extracellular deposition of beta-amyloid peptide (Abeta) in cerebral plaques. Abeta is derived from the beta-amyloid precursor protein (APP) by the enzymes alpha-, beta- and gamma-secretase. Compounds that enhance alpha-secretase, but inhibit beta- or
D Aguado-Llera et al.
Journal of neurochemistry, 92(3), 607-615 (2005-01-22)
Insulin-like growth factor-I (IGF-I) has protective effects against beta-amyloid (Abeta)-induced neuronal cell death. Because alterations of the somatostatinergic system have been described in Alzheimer's disease, we investigated the effects of the Abeta peptide and the possible protective role of IGF-I
Maricarmen Hernández-Rodríguez et al.
Protein science : a publication of the Protein Society, 22(10), 1320-1335 (2013-08-02)
The formation of fibrils and oligomers of amyloid beta (Aβ) with 42 amino acid residues (Aβ 1-42 ) is the most important pathophysiological event associated with Alzheimer's disease (AD). The formation of Aβ fibrils and oligomers requires a conformational change
Ana Martinho et al.
The FEBS journal, 277(16), 3427-3436 (2010-07-22)
Transthyretin (TTR), an amyloid-beta (Abeta) scavenger protein, and metallothioneins 2 and 3 (MT2 and MT3), low molecular weight metal-binding proteins, have recognized impacts in Abeta metabolism. Because TTR binds MT2, an ubiquitous isoform of the MTs, we investigated whether it
S Cantara et al.
FEBS letters, 581(4), 702-706 (2007-02-06)
Oxidative stress is one of the factor contributing to blood brain barrier degeneration. This phenomenon is observed during pathological conditions such as Alzheimer's disease or cerebral amyloid angiopathy in which brain haemorrhages are very frequent. Both diseases are characterized by
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