Anti-Aquaporin-4 Antibody

Aquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel (MIWC), WCH4) is encoded by the Aqp4 gene (Gene ID: 25293) in rat. Aquaporin-4 is a transmembrane water channel protein predominantly expressed in astrocytes within the central nervous system (CNS), where it plays a critical role in maintaining water homeostasis and supporting key physiological processes. Strategically localized at the perivascular end-feet of astrocytes, Aquaporin-4 facilitates bidirectional water transport across the blood-brain barrier and is integral to the glymphatic system, a clearance pathway responsible for removing interstitial solutes such as amyloid-beta. Dysregulation of Aquaporin-4 has been implicated in numerous neuropathological conditions, including neuromyelitis optica spectrum disorder (NMOSD), Alzheimer′s disease, traumatic brain injury, and brain edema. In NMOSD, autoantibodies targeting Aquaporin-4 lead to astrocyte destruction, inflammation, and central nervous system damage, emphasizing the importance of early and accurate detection methods such as biosensors. Aquaporin-4 isoforms, particularly M23, form supramolecular structures called orthogonal arrays of particles (OAPs), which are crucial for its localization and function. Extended isoforms such as AQP4ex further regulate water channel activity and contribute to amyloid-beta clearance, highlighting their therapeutic potential in neurodegenerative diseases. Redistribution of Aquaporin-4 within astrocytes, often due to disruptions in its anchoring dystrophin-associated protein complex, has been associated with impaired glymphatic function, exacerbating conditions like idiopathic normal pressure hydrocephalus and glioblastoma. (Ref.: Silverglate, B., et al., (2023), Expert Opin.Therap. Targets, 27(7), 523-530; Peng, S., et al., (2023), Neurobiol. Dis. 179, Article 106035; Vandebroek, A., and Yasui, M. (2020), Int. J. Mol. Sci. 21(5); 1603).

Target molecular weight ~32 kDa observed. 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

This rabbit polyclonal antibody detects Aquaporin-4. It targets an epitope within 16 amino acids from the C-terminal cytoplasmic domain.

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal cytoplasmic domain of human.

Anti-Aquaporin-4, Cat. No. AB2218 is a rabbit polyclonal antibody that detects Aquaporin-4 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aquaporin-4 in rat brain tissue lysate.




Tested applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aquaporin-4 in human and mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin-4 in human, rat, and mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Neuroscience

Replaces: AB3068

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Control
Tested on adult rat brain lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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