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Merck

AB5605

Anti-4-Hydroxynonenal Antibody

serum, Chemicon®

Synonym(s):

4-HNE

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

dry ice

target post-translational modification

unmodified

General description

The peroxidation of membrane lipids by free radicals is a well-controlled, ongoing process that occurs during normal cell turnover and the normal aging process. There is also the uncontrolled process of free radical production and increased lipid peroxidation that is associated with hepatic injury as a consequence of exposure to halogenated hydrocarbons, excessive alcohol abuse, and acute or chronic iron overload. Excess free radical production and lipid peroxidation is thought to be an etiological factor in such diseases as atherosclerosis, diabetes, genetic hemochromatosis, cancer and neurodegenerative diseases, such as Alzheimer′s and Parkinson′s disease.4-Hydroxynonenal (HNE) is a highly reactive, cytotoxic aldehyde that is released during the oxidation of w-6-unsaturated fatty acids. HNE can efficiently react with sulfhydryl groups or histidine and lysine groups of proteins to form stable HNE-protein adducts, in addition to phospholipids and nucleic acids. It is through this HNE-modification of biomolecules that HNE is thought to exert its cytotoxic effects. This multifunctional molecule may be considered as a "second toxic messenger," which disseminates and augments initial free radical events. It is believed to be largely responsible for the cytopathological effects observed during oxidative stress. High levels of 4-HNE have been detected in a large number of disease states, indicating some involvement of this aldehyde in their pathogenesis. Some of the prominent pathobiochemical effects of 4-HNE are its stimulation of fibrogenesis and inflammation. This indicates a potential contribution to the pathogenesis of several chronic diseases, the progression of which is supported by inflammatory reactions and characterized by fibrosis. 4-HNE may also play a role in carcinogenesis due to its ability to modulate cell proliferation through interference with the activity of cyclins and protein kinases and inhibiting the apoptotic response.

Immunogen

4-HNE.

Application

Anti-4-Hydroxynonenal Antibody detects level of 4-Hydroxynonenal & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western Blot: 1:3000 on human alzheimer′s Disease brain lysate.
ELISA: >1:10,000

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Specific for 4-Hydroxynonenal (4-HNE).
The HNE moiety recognized by this AB5605 antibody is a biochemical modification independent of species. The anit-4-HNE antibody should have wide species reactivity accordingly.

Physical form

Goat antiserum. Liquid with 0.1% sodium azide

Preparation Note

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erythrocyte shape abnormalities, membrane oxidative damage, and ?-actin alterations: an unrecognized triad in classical autism.
Ciccoli, L; De Felice, C; Paccagnini, E; Leoncini, S; Pecorelli, A; Signorini et al.
Mediators of Inflammation null
Redox imbalance and morphological changes in skin fibroblasts in typical Rett syndrome.
Signorini, C; Leoncini, S; De Felice, C; Pecorelli, A; Meloni, I; Ariani, F; Mari et al.
Oxidative Medicine and Cellular Longevity null
Claudio De Felice et al.
Neurobiology of disease, 68, 66-77 (2014-04-29)
Rett syndrome (RTT) is a rare neurodevelopmental disorder affecting almost exclusively females, caused in the overwhelming majority of the cases by loss-of-function mutations in the gene encoding methyl-CpG binding protein 2 (MECP2). High circulating levels of oxidative stress (OS) markers
Bart Cortjens et al.
PloS one, 11(12), e0168779-e0168779 (2016-12-23)
The human pneumovirus respiratory syncytial virus (RSV) is the most common pathogen causing lower respiratory tract disease in young children worldwide. A hallmark of severe human RSV infection is the strong neutrophil recruitment to the airways and lungs. Massive neutrophil
Effects of ?-3 PUFAs supplementation on myocardial function and oxidative stress markers in typical Rett syndrome.
Maffei, S; De Felice, C; Cannarile, P; Leoncini, S; Signorini, C; Pecorelli, A; Montomoli et al.
Mediators of Inflammation null
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Related Content

White Paper- Modern Methods in Oxidative Stress Research

"Redox reactions are powerful chemical processes that involve the reduction and oxidation of proteins and metabolites found in living things. The mechanisms that regulate them are key to maintaining homeostasis and the balance between good health and disease pathology. Oxidative stress is the state where the delicate balance of redox biology is upset, and the pathology of oxidative stress are the cellular consequences to such an imbalance."

GOAT ANTI-4-HYDROXYNONENAL
Pathways and Biomarkers of Oxidative Stress

"Aging: getting older, exhibiting the signs of age, the decline in the physical (and mental) well-being over time, leading to death. Since the beginning of time, man has been obsessed with trying to slow down, stop, or even reverse the signs of aging. Many have gone as far as experimenting with nutritional regimens, eccentric exercises, fantastic rituals, and naturally occurring or synthetic wonder-elements to evade the signs of normal aging. Biologically speaking, what is aging? And what does the latest research tell us about the possibility of discovering the elusive “fountain of youth”? Many advances in our understanding of aging have come from systematic scientific research, and perhaps it holds the key to immortality. Scientifically, aging can be defined as a systems-wide decline in organismal function that occurs over time. This decline occurs as a result of numerous events in the organism, and these events can be classified into nine “hallmarks” of aging, as proposed by López-Otin et al. (2013). Several of the pathologies associated with aging are a direct result of these events going to extremes and may also involve aberrant activation of proliferation signals or hyperactivity. The hallmarks of aging have been defined based on their fulfillment of specific aging related criteria, such as manifestation during normal aging, acceleration of aging if experimentally induced or aggravated, and retardation of aging if prevented or blocked, resulting in increased lifespan. The nine hallmarks of aging are genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. The biological processes underlying aging are complex. By understanding the hallmarks in greater detail, we can get closer to developing intervention strategies that can make the aging process less of a decline, and more of a recline."

Global Trade Item Number

SKUGTIN
AB560504053252331862

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