AB5840
Anti-Dab1 Antibody
serum, Chemicon®
Synonym(s):
Disabled homolog 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
80 kDa
Dab1 is a protein arising from the mouse gene dab, that is related to the Drosophila gene ′disabled′. Mutations in the mouse dab gene may result in the ′scrambler′ and ′yotari′ phenotypes. Dab encodes a phosphoprotein that binds non-receptor tyrosine kinases and has been implicated in neuron development. Dab1 is expressed in neuronal populations exposed to reelin, as determined by in situ hybridization to embryonic mouse brain tissue. Dab1 may function as a signaling molecule that regulates positioning in the developing brain. Cloning of human DAB1 and sequence determinations suggests a 96% homology to the mouse sequence. Very low density lipoprotein receptors (VLDLR) and apolipoprotein E receptor-2 (APOER2) both bind Dab1 on their cytoplasmic tails. Dab1 expression is upregulated in knockout mice lacking both Vldlr and Apoer2 genes.
Immunogen
Highly purified GST-fusion protein corresponding to residues 400-555 of mouse Dab1.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Signaling Neuroscience
Developmental Neuroscience
Signaling Neuroscience
This Anti-Dab1 Antibody is validated for use in IC, IH, IP, WB for the detection of Dab1.
Western blotting: 1:1,000-1:5,000
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:5,000-1:20,000 on cryofixed tissues
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:5,000-1:20,000 on cryofixed tissues
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
On western blots of mouse brain extracts, the antibody recognizes a band migrating at approximately 80 kDa corresponding to Dab1. No cross reactivity to human or mouse Dab2.
Physical form
Delipidated and defibrinated Serum in 0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.
Serum, delipidated and defibrinated
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABS167
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Reelin, integrin and DAB1 interactions during embryonic cerebral cortical development.
Schmid, RS; Jo, R; Shelton, S; Kreidberg, JA; Anton, ES
Cerebral Cortex (1991)
Shiro Suetsugu et al.
The Biochemical journal, 384(Pt 1), 1-8 (2004-09-14)
Migration of cells is critical to development of the central nervous system. Reelin, which was identified from the reeler mutant mice having a defect in the multilamellar structure of the brain, is thought to be a key signalling molecule that
Nicholas J Bradshaw et al.
Schizophrenia research, 215, 506-513 (2017-04-24)
Disrupted in Schizophrenia 1 (DISC1) is a prominent gene in mental illness research, encoding a scaffold protein known to be of importance in the developing cerebral cortex. Reelin is a critical extracellular protein for development and lamination of the prenatal
Takahiro Fujimoto et al.
Biochemical and biophysical research communications, 407(2), 438-443 (2011-03-23)
Three inositol 1,4,5-trisphosphate receptor (IP(3)R) subtypes are differentially expressed among tissues and function as the Ca(2+) release channel on specialized endoplasmic reticulum (ER) membranes. The proper subcellular localization of IP(3)R is crucial for its proper function, but this molecular mechanism
Eric Zluhan et al.
eNeuro, 7(4) (2020-07-10)
Disruptions in neuronal dendrite development alter brain circuitry and are associated with debilitating neurological disorders. Nascent apical dendrites of cortical excitatory neurons project into the marginal zone (MZ), a cell-sparse layer characterized by intense chondroitin sulfate proteoglycan (CSPG) expression. Paradoxically
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