Anti-EIF4A2 Antibody

Eukaryotic initiation factor 4A-II, (EIF4A2), or ATP-dependent RNA helicase eIF4A-2 is the critical RNA helicase protein required for mRNA cap recognition and mRNA binding to the ribosome. EIF4A2 is part of a multi-subunit complex that allows translation of mRNAs to begin. EIF4A2 unwinds the RNA secondary structure in the 5’-UTR region and thus allows the small ribosomal subunit to bind the mRNA and begin scanning for the initiator codon. EIF4A2 also plays a key role in mRNA expression inhibition. Recent research demonstrates that inhibition of EIF4A2 function via microRNA inhibition is required first, which then leads to mRNA destabilization and ultimately translation suppression. EIF4A2 is expressed in all tissues, as well as in cancerous tumors. Expression is very high in ovary, followed by liver, kidney, brain and pancreas in lower amounts. EIF4A2 is often used as a “housekeeping” marker as well because of its wide expression.

~46 kDa observed

GST-tagged fusion protein corresponding to human EIF4A2.

Anti-EIF4A2 Antibody is a highly specific rabbit polyclonal antibody, that targets EIF4A2 & has been tested in western blotting & Immunofluorescence.

Immunofluorescence Analysis: A 1:25 dilution from a representative lot detected EIF4A2 in HepG2 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected EIF4A2 in MCF7 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.