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AM34

Sigma-Aldrich

Anti-Caspase-3 (Ab-2) Mouse mAb (10C1.C9)

liquid, clone 10C1.C9, Calbiochem®

Synonym(s):

Anti-CPP32, Anti-YAMA, Anti-Apopain, Anti-CASP3, Anti-LICE, Anti-ICE-3, Anti-YAMA, Anti-Apopain, Anti-CASP3, Anti-LICE, Anti-ICE-3, Anti-CPP32

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody product type

primary antibodies

clone

10C1.C9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes the ~32, ~28, and ~17 kDa forms of caspase-3. Additional unrelated proteins between ~60 and ~45 kDa may also be detected.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~32, ~28, and ~17 kDa forms of caspase-3.

Immunogen

full-length, recombinant, human caspase-3

Application

Immunoblotting (1 µg/ml)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Immunoprecipitation (not recommended)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Other Notes

Additional unrelated proteins between ~45 and ~60 kDa may also be detected. Antibody should be titrated for optimal results in individual systems.



Recommended protocol for extracting CPP32 from cell pellets.

1. Rinse cells 2 times with cold PBS and resuspend the final cell pellet (6 x 105 cells/µl) in 50 mM PIPES/KOH pH 6.5, 2 mM EDTA, 0.1% CHAPS, 5 mM DTT, 20 µg/ml leupeptin, 10 µg/ml pepstatin A, 10 µg/ml aprotinin, and 2 mM PMSF.

2. Subject the cells to 3 freeze/thaw cycles in dry ice/methanol.

3. Centrifuge the lysate at 4°C for 30 min at 20,000 x g and recover the supernatant fraction.

4. Apply a minimum of 25 µg protein per lane (the amount may need to be optimized for individual cell lines or sample types).
Alnemri, E.S. 1997. J. Cell. Biochem.64, 33.
Bayaert, R., et al. 1997. J. Biol. Chem.272, 11694.
Erhardt, P., et al. 1997. J. Biol. Chem.272, 15049.
Inayat-Hussain, S.H., et al. 1997. Hepatology25, 1516.
Krjewska, M., et al. 1997. Cancer Res.57, 1605.
Mashima, T., et al. 1997. Oncogene14, 1007.
McConnell, K.R., et al. 1997. J. Immunol.158, 2083.
Posmantur, R., et al. 1997. J. Neurochem.68, 2328.
Suzuki, A., et al. 1997. Exp. Cell. Res.233, 48.
Liu, X., et al. 1996. J. Biol. Chem.271, 13371.
Quan, L.T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 1972.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Tiso, N., et al. 1996. Biochem. Biophys. Res. Commun.225 983.
Xue, D., et al. 1996. Genes Dev.10, 1073.
Tewari, M., et al. 1995. Cell81, 801.
Fernandes-Alnemri, T., et al. 1994. J. Biol. Chem.269, 30761.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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